SmaI can be used at 37☌ for brief incubations.Įfficient cleavage requires at least two copies of the NarI recognition sequence.Įfficient cleavage requires at least two copies of the PluTI recognition sequence. Prolonged incubation with BseRI may lead to degradation of the DNA.Ĭleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. Sticky ends from different BbsI sites may not be compatible. PaeR7I does not recognize the sequence CTCTCGAG.ĮcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.Īfter cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. Sticky ends from different EcoO109I sites may not be compatible. Prolonged incubation with NdeI may lead to removal of additional nucleotides. Sticky ends from different Bpu10I sites may not be compatible.
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